Journal
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 20, Issue 6, Pages 4078-4084Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-012-1356-4
Keywords
trzN gene; 16S rRNA gene; Biomass; Bioaugmentation; Bioremediation; Herbicide; Carbon source; Microbial community
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Funding
- State Key Laboratory of Ecohydraulic Engineering in Shaanxi
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The objective of this study was to investigate the impact of glucose supplementation on the soil microbiota inoculated with the atrazine-degrading Arthrobacter strain DAT1. Soil microcosms with different treatments were constructed for biodegradation tests. The impact of glucose supplementation on atrazine degradation capacity of the strain DAT1 and the strain's survival and growth were assessed. The densities of the 16S rRNA gene and the atrazine-metabolic trzN gene were determined using quantitative PCR. The growth of the strain DAT1 and the bacterial community structure were characterized using terminal restriction fragment length polymorphism. Glucose supplementation could affect atrazine degradation by the strain DAT1 and the strain's trzN gene density and growth. The density of the16S rRNA gene decreased during the incubation period. Glucose supplementation could alter the bacterial community structure during the bioaugmentation process. Glucose supplementation could promote the growth of the autochthonous soil degraders that harbored novel functional genes transforming atrazine. Further study will be necessary in order to elucidate the impact of exogenous carbon on autochthonous and inoculated degraders. This study could add some new insights on atrazine bioremediation.
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