Journal
JOURNAL OF RAMAN SPECTROSCOPY
Volume 46, Issue 7, Pages 597-604Publisher
WILEY
DOI: 10.1002/jrs.4713
Keywords
beta-carotene; quantitative SERS; Ag nanoparticles; Adriatic sea urchin
Categories
Funding
- Government of the Republic of Croatia [5/2014]
- Ministry of Science, Education and Sport (MSES)
- Marie Curie FP7-PEOPLE-2011-COFUND
- Sectorial Operational Program for Human Resources Development
- European Social Fund [POSDRU/159/1.5/S/132400]
Ask authors/readers for more resources
While recording SERS spectra of pure -carotene at sub-micromole concentrations for reference purpose, we discovered an unusual spectral response never reported before. In pre-resonance conditions with the 532-nm line, SERS of -carotene with AgNPs exhibits among the strong upsilon(CC) mode at 1512cm(-1) unshifted from normal Raman spectrum, additional strong bands at 1649, 1575 and 1387cm(-1) as well as other medium bands not observed in the Raman spectrum of the crystalline powder. Such behavior is explained in terms of selection rules relaxation upon cyclohexene terminal rings of the -carotene interaction with the NP surface. AFM images of the SERS system suggested dimers and trimers clustering of the nanoparticles with adsorbed -carotene. In light of the new SERS feature the consequences in correct interpretation of the SERS imaging from complex biosystems containing carotenoids are discussed. Relative intensity ratio of the -carotene band at 1512cm(-1) and water against concentration allowed a reliable SERS calibration curve for 50 to 500nmoll(-1) concentration range and provided quantitative SERS assessment of the carotenoid content in the sea urchin (Paracentrotus lividus) gonads extracts. Copyright (c) 2015 John Wiley & Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available