Journal
ENVIRONMENTAL POLLUTION
Volume 185, Issue -, Pages 333-339Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2013.10.016
Keywords
Free and covalently bound microcystins; Complex matrices; Lemieux oxidation; LC/MS-MS; NF V03 110 guideline
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Funding
- General Directorate of Health, Ministry of Public Health through the Bioaccess Project
- ANSES EST [2010/2/002]
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So far only a few publications have explored the development of extraction methods of cyanotoxin extracted from complex matrices. With regard to cyanobacterial microcystins (MCs), the data on the contamination of the flesh of aquatic organisms is hard to compare and very limited due to the lack of validated methods. In recent years, evidence that both free and bound fractions of toxin are found in these tissues has highlighted the need to develop effective methods of quantification. Several techniques do exist, but only the Lemieux oxidation has so far been used to investigate complex tissue matrices. In this study, protocols based on the Lemieux approach were adapted for the quantitative chemical analysis of free MC-LR and MMPB derived from bound toxin in the tissues of juvenile trout gavaged with MC-LR. Afterwards, the NF V03 110 guideline was used to characterize the protocols elaborated and evaluate their effectiveness. (C) 2013 Published by Elsevier Ltd.
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