Journal
ENVIRONMENTAL POLLUTION
Volume 164, Issue -, Pages 28-35Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2012.01.018
Keywords
Xenoestrogens; Environmental mixtures; Steroidogenesis; Zebrafish; Transcription factors
Categories
Funding
- FEDER funds through the Competitiveness and Trade Expansion Program - COMPETE
- FCT - Fundacao para a Ciencia e a Tecnologia [PTDC/MAR/70436/2006]
- Fundação para a Ciência e a Tecnologia [PTDC/MAR/70436/2006] Funding Source: FCT
Ask authors/readers for more resources
In natural environments fish are exposed to endocrine disrupting compounds (EDCs) present at low concentrations and with different modes of actions. Here, adult zebrafish of both sexes were exposed for 21 days to an estrogenic mixture (Mix) of eleven EDCs previously quantified in Douro River estuary (Portugal) and to 100 ng/L 17 alpha-ethinylestradiol (EE2) as positive control. Vitellogenin mRNA and HSI in males confirmed both exposure regimes as physiologically active. Potential candidates for estrogenic disturbance of steroidogenesis were identified (StAR, 17 beta-HSD1, cyp19a1), but Mix only affected cyp19a1 in females. Significant differences in the response of FSH beta, cypa19a2, 20 beta-HSD were observed between EE2 and Mix. Mtf-1 and tfap2c transcription factor binding sites were discovered in the putative promoter regions and corresponding transcription factors were found to be differentially expressed in response to Mix and EE2. The results suggest that non-classical effects of estrogenic EDC in fish are mediated via transcription factors. (C) 2012 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available