Effect of different arbuscular mycorrhizal fungal isolates on growth and arsenic accumulation in Plantago lanceolata L.

The role of indigenous and non-indigenous arbuscular mycorrhizal fungi (AMF) on As uptake by Plantago lanceolate L growing on substrate originating from mine waste rich in As was assessed in a pot experiment. P. lanceolata inoculated with AMF had higher shoot and root biomass and lower concentrations of As in roots than the non-inoculated plants. There were significant differences in As concentration and uptake between different AMF isolates. Inoculation with the indigenous isolate resulted in increased transfer of As from roots to shoots; AMF from non-polluted area apparently restricted plants from absorbing As to the tissue; and plants inoculated with an AMF isolate from Zn-Pb waste showed strong As retainment within the roots. Staining with dithizone indicated that AMF might be actively involved in As accumulation. The mycorrhizal colonization affected also the concentration of Cd and Zn in roots and Pb concentration, both in shoots and roots. (c) 2012 Elsevier Ltd. All rights reserved.