Journal
ENVIRONMENTAL POLLUTION
Volume 158, Issue 9, Pages 2872-2879Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2010.06.020
Keywords
Pyrene; nidA; qPCR; Mycobacterium; T-RFLP; Bacterial community
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Funding
- Natural Science Foundation of China [40801118]
- Ministry of Science and Technology [2007AA061101, 2009DFB90120]
- CAS/SAFEA [KZCX2-Yw-T08]
- Natural Environment Research Council [ceh010010] Funding Source: researchfish
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This study investigates the dynamics of pyrene degradation rates, microbial communities, and functional gene copy numbers during the incubation of pyrene-spiked soils. Spiking pyrene to the soil was found to have negligible effects on the bacterial community present. Our results demonstrated that there was a significant difference in nidA gene copy numbers between sampling dates in QZ soil. Mycobacterium 16S rDNA clone libraries showed that more than 90% mycobacteria detected were closely related to fast-growing PAH-degrading Mycobacterium in pyrene-spiked soil, while other sequences related to slow-growing Mycobacterium were only detected in the control soil. It is suggested that nidA gene copy number and fast-growing PAH-degrading Mycobacterium could be used as indicators to predict pyrene contamination and its degradation activity in soils. (C) 2010 Elsevier Ltd. All rights reserved.
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