Differential Regulation of Dopamine Transporter Function and Location by Low Concentrations of Environmental Estrogens and 17β-Estradiol

BACKGROUND: The effects of 17 beta-estradiol (E-2) and xenoestrogens (XEs) on dopamine transport may have important implications for the increased incidence of neurologic disorders, especially in women during life stages characterized by frequent hormonal fluctuations. OBJECTIVE: We examined low concentrations of XEs [dieldrin, endosulfan, o',p'-dichlorodiphenyl-ethylene (DDE), nonylphenol (NP), and bisphenol A (BPA)] for nongenomic actions via action of membrane estrogen receptors (ERs). METHODS: We measured activity of the dopamine transporter (DAT) by the efflux of H-3-dopamine in nontransfected nerve growth factor-differentiated PC12 rat pheochromocytoma cells expressing membrane DAT, ER-alpha, ER-beta, and G-protein-coupled receptor 30. We used a plate immunoassay to monitor trafficking of these proteins. RESULTS: All compounds at 1 nM either caused efflux or inhibited efflux, or both; each compound evoked a distinct oscillatory pattern. At optimal times for each effect, we examined different concentrations of XEs. All XEs were active at some concentration < 10 nM, and dose responses were all nonmonotonic. For example, 10(-14) to 10(-11) M DDE caused significant efflux inhibition, whereas NP and BPA enhanced or inhibited efflux at several concentrations. We also measured the effects of E-2/XE combinations; DDE potentiated E-2-mediated dopamine efflux, whereas BPA inhibited it. In E-2-induced efflux, 15% more ER-alpha trafficked to the membrane, whereas ER-beta waned; during BPA-induced efflux, 20% more DAT was trafficked to the plasma membrane. CONCLUSIONS: Low levels of environmental estrogen contaminants acting as endocrine disruptors via membrane ERs can alter dopamine efflux temporal patterning and the trafficking of DAT and membrane ERs, providing a cellular mechanism that could explain the disruption of physiologic neurotransmitter function.