Journal
ENVIRONMENTAL ENTOMOLOGY
Volume 38, Issue 3, Pages 686-699Publisher
OXFORD UNIV PRESS INC
DOI: 10.1603/022.038.0320
Keywords
Anoplophora glabripennis; gut microbiota; host tree resistance; culture-independent community analysis
Categories
Funding
- USDA-NRI-CRSEES [2008-35504-04464, 2009-35302-05286]
- Alphawood Foundation, Chicago
- Pennsylvania State University College of Agricultural Sciences Seed
Ask authors/readers for more resources
Anoplophora glabripennis, the Asian longhorned beetle, is a wood-boring insect that call develop in a wide range of healthy deciduous hosts and requires gut microbes to aid ill wood degradation and digestion. Here we show that larval A. glabripennis harbor a diverse gut bacterial community, and this community call be extremely variable when reared in different host trees. A. glabripennis reared in a preferred host (Acer saccharum) had the highest gut bacterial diversity compared with larvae reared either in a secondary host (Quercus palustris), a resistant host (Pyrus calleryana), or on artificial diet. The gut microbial community of larval A. glabripennis collected from field populations on Brooklyn, NY, showed the highest degree of complexity among all samples in this study. Overall, when larvae fed on a preferred host, they hat-bored a broad diversity of gut bacteria spanning the alpha-, beta-, gamma-Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Cellulase activities (beta-1,4-endoglucanase, beta-1,4-exoglucanase, and beta-1,4-glucosidase) in the guts of larvae fed in a preferred host (A. saccharum) or a secondary host (Q. palustris) were significantly higher than that of artificial diet fed larvae. Larvae that fed on wood from a resistant host (P. calleryana) showed suppressed total gut cellulase activity. Results show that the host tree can impact both gut microbial community complexity and cellulase activity in A. glabripennis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available