Journal
JOURNAL OF PROTEOMICS
Volume 119, Issue -, Pages 90-99Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2015.01.017
Keywords
Cerebrospinal fluid; Proteome; Functional annotation
Categories
Funding
- Beijing Municipal Health System 215 High-level Medical Personnel Culturing Project [2011-3-038]
- Foundation of Science and Technology for Excellent Students Abroad of Beijing City
- Natural Science Foundation of Beijing City [7132094]
- Multidisciplinary Cooperation Project of the Beijing Nova program [xxhz201409]
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Knowledge about the normal human cerebrospinal fluid (CSF) proteome serves as a baseline reference for CSF biomarker discovery and provides insight into CSF physiology. In this study, high-pH reverse-phase liquid chromatography (hp-RPLC) was first integrated with a TripleTOF 5600 mass spectrometer to comprehensively profile the normal CSF proteome. A total of 49,836 unique peptides and 3256 non-redundant proteins were identified. To obtain high-confidence results, 2513 proteins with at least 2 unique peptides were further selected as bona fide CSF proteins. Nearly 30% of the identified CSF proteins have not been previously reported in the normal CSF proteome. More than 25% of the CSF proteins were components of CNS cell microenvironments, and network analyses indicated their roles in the pathogenesis of neurological diseases. The top canonical pathway in which the CSF proteins participated was axon guidance signaling. More than one third of the CSF proteins (788 proteins) were related to neurological diseases, and these proteins constitute potential CSF biomarker candidates. The mapping results can be freely downloaded at http://122.70.220.102:8088/csf/, which can be used to navigate the CSF proteome. Biological significance This study identified and functionally annotated the largest high-precision dataset of the CSF proteome, which offers a baseline reference for CSF biomarker discovery and reveals insight into CSF physiology. (C) 2015 Elsevier B.V. All rights reserved.
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