Journal
JOURNAL OF PROTEOMICS
Volume 126, Issue -, Pages 172-188Publisher
ELSEVIER
DOI: 10.1016/j.jprot.2015.05.030
Keywords
Glycomics; Insects; Mass spectrometric; Glycans; Oligosaccharides; HPLC
Categories
Funding
- FWF (Austrian Science Fund) [P21946, P25058]
- FWF [W1224]
- Swedish Research Council [20135895, 2010-5322, 342-2004-4434]
- Knut and Alice Wallenberg Foundation
- National Institute of General Medical Sciences of the National Institutes of Health [P41GM103490]
- Austrian Science Fund (FWF) [P 21946, P 25058] Funding Source: researchfish
- Austrian Science Fund (FWF) [P25058] Funding Source: Austrian Science Fund (FWF)
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Mosquitoes are important vectors of parasitic and viral diseases with Anopheles gambiae transmitting malaria and Aedes aegypti spreading yellow and Dengue fevers. Using two different approaches (solid-phase extraction and reversed-phase or hydrophilic interaction HPLC fractionation followed by MALDI-TOF MS or permethylation followed by NSI-MS), we examined the N-glycans of both A. gambiae and A. aegypti larvae and demonstrate the presence of a range of paucimannosidic glycans as well as bi- and tri-antennary glycans, some of which are modified with fucose or with sulphate or glucuronic acid residues; the latter anionic modifications were also found on N-glycans of larvae from another dipteran species (Drosophila melanogaster). The sulphate groups are attached primarily to core alpha-mannose residues (especially the alpha 1,6-linked mannose), whereas the glucuronic add residues are linked to non-reducing beta 1,3-galactose. Also, O-glycans were found to possess glucuronic acid and sulphate as well as phosphoethanolamine modifications. The presence of sulphated and glucuronylated N-glycans is a novel feature in dipteran glycomes; these structures have the potential to act as additional anionic glycan ligands involved in parasite interactions with the vector host. (C) 2015 Elsevier B.V. All rights reserved.
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