Journal
JOURNAL OF PROTEOME RESEARCH
Volume 14, Issue 9, Pages 3583-3594Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.5b00435
Keywords
Chromosome-Centric Human Proteome Project; testis; missing proteins; proteome; transcriptome; individual
Categories
Funding
- Chinese National Basic Research Programs [2011CB910600, 2013CB911201, 2014CBA02002-A]
- National Natural Science Foundation of China [31400697, 31170780, 31470809, 31400698]
- International Collaboration Program [2014DFB30020]
- National High-Tech Research and Development Program of China [2012AA020202, 2012AA020502, 2011AA02A114, 2014AA020900, 2014AA020607]
- National Natural Science Foundation of Beijing [5152008]
- National Mega projects for Key Infectious Diseases [2013zx10003002]
- Key Projects in the National Science & Technology Pillar Program [2012BAF14B00]
- Fundamental & Advanced Research Project of Chongqing, China [cstc2013jcyjC00001]
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Investigations of missing proteins (MPs) are being endorsed by many bioanalytical strategies. We proposed that proteogenomics of testis tissue was a feasible approach to identify more MPs because testis tissues have higher gene expression levels. Here we combined proteomics and transcriptomics to survey gene expression in human testis tissues from three post-mortem individuals. Proteins were extracted and separated with glycine- and tricine-SDS-PAGE. A total of 9597 protein groups were identified; of these, 166 protein groups were listed as MPs, including 138 groups (83.1%) with transcriptional evidence. A total of 2948 proteins are designated as MPs, and 5.6% of these were identified in this study. The high incidence of MPs in testis tissue indicates that this is a rich resource for MPs. Functional category analysis revealed that the biological processes that testis MPs are mainly involved in are sexual reproduction and spermatogenesis. Some of the MPs are potentially involved in tumorgenesis in other tissues. Therefore, this proteogenomics analysis of individual testis tissues provides convincing evidence of the discovery of MPs. All mass spectrometry data from this study have been deposited in the ProteomeXchange (data set identifier PXD002179).
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