Journal
ENGINEERING IN LIFE SCIENCES
Volume 9, Issue 1, Pages 66-73Publisher
WILEY
DOI: 10.1002/elsc.200700040
Keywords
Aspartic protease; Solid-state fermentation; Solvent-stable enzyme; Tannery solid waste
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Funding
- Council of Scientific and Industrial Research (CSIR)
- India Central Leather Research Institute (CLRI), India)
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Synergistes sp. DQ560074 produced a protease in submerged fermentation (SmF) at 400-420 U/mL and in solid-state fermentation (SSF) at 745-755 U/g. The protease, which belongs to the aspartic protease class, was active over a wide range of pH (5-7) and at high temperatures (25-45 degrees C). The protease is stable and active in various polar protic solvents (50% v/v) like ethanol, isopropanol, n-butanol, in polar aprotic solvents (50%v/v) like acetonitrile, and in non-polar solvents (50%v/v) such as ethylacetate and toluene, but not in hydrophilic organic solvents (methyl alcohol and acetone). As far as we know, this is the first contribution to the production of a mesophilic protease with solvent stability in SSF using a proteinaceous solid waste.
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