Journal
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA
Volume 31, Issue 6, Pages 385-388Publisher
EDICIONES DOYMA S A
DOI: 10.1016/j.eimc.2012.09.005
Keywords
Extended-spectrum beta-lactamase; CTX-M-15; ST131; O25B:H4 Escherichia coli
Categories
Funding
- Ministerio de Ciencia e Innovacion (Instituto de Salud Carlos III, Fondo de Investigacion Sanitaria) [REIPI- RD06/0008-1018, PS09/01273, PI10/01955, PI070190]
- Ministerio de Educacion y Ciencia [AGL-2008-02129]
- Junta de Andalucia [PI-0048/2008, P09-CTS-5259, PI 0034-2009]
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Introduction: A multiresistant CTX-M-15-producing clonal group of Escherichia coli isolates, namely O25b:H4/ST131, has recently emerged in three continents. At this moment, appropriate studies to assess the real prevalence of this successful lineage are still scarce. Methods: In a prospective study in the south of Spain, among all clinical E. Coli isolates recovered in Seville during a 30 week period in 2010, ST131 was screened by using PCR for O25b/pabB3/B23 traits. ESBL enzymes were characterized by PCR and sequencing. Genetic relatedness was performed by XbaI PFGE. Results: This clonal group was found to be prevalent (12.5% of all E. coli isolates), and only 37(6.8% of ST131 isolates) were ESBL producers. Among 25 characterized ESBL-producing ST131 isolates, 96% harbored CTX-M-15. ST131 isolates were more frequently resistant to amoxicillin/clavulanate, aminoglycosides and fluoroquinolones in both ESBL and non-ESBL producers groups. Xbal PFGE performed on 88 ST131 isolates showed three pulsotypes, which included >= 4 isolates each (25% of all typed ST131 isolates), and 11 pulsotypes, which contained 2-3 isolates each. Three of 14 pulsotypes of this clonal group included both nalidixic acid-resistant and susceptible isolates, and five pulsotypes included both ESBL and non-ESBL producers. Conclusions: Our findings suggest that O25b/ST131 is a prevalent clone in our area, and the observed prevalence of ESBL-producers within this clone is similar to that found in the total isolates of this species. Certain pulsotypes among ST131 clone that showed a greater expansion, and ESBL genes acquisition or quinolone resistance could explain part of this prevalence. (C) 2012 Elsevier Espana, S.L. All rights reserved.
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