Ca2+ determines the entropy changes associated with the formation of transition states during water oxidation by Photosystem II

The role of Ca2+ in the Photosystem II water oxidation mechanism has been investigated in the higher redox states of the enzyme. The involvement of Ca2+ in structuring the environment of the tyrosine Y-Z in the S-3 state has been investigated by studying the effect of a Ca/Sr exchange by pulse-EPR spectroscopy. It is shown that Ca and Yz are involved in a common hydrogen bond network in S-3. The comparison of the temperature dependence of the rate of the water oxidation reaction shows that the slowdown of the S3YZ center dot -> S-0 transition in Sr-PSII mainly arises from a decrease of the entropic part of the Delta G(#) of the reaction. This suggests that Ca/Sr exchange perturbs the distribution of the conformational microstates and thereby hinders the overall water splitting process.