Journal
ENERGY
Volume 78, Issue -, Pages 9-15Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.energy.2014.06.009
Keywords
Biomass; Diatom; Irradiation mutation; Lipid; Salinity domestication
Categories
Funding
- National Natural Science Foundation of China [51176163]
- National High Technology R&D Program of China [2012AA050101]
- International Sci. & Tech. Cooperation Program of China [2012DFG61770]
- Zhejiang Provincial Natural Science Foundation of China [LR14E060002]
- Program for New Century Excellent Talents in University [NCET-11-0446]
- Specialized Research Fund for the Doctoral Program of Higher Education [20110101110021]
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To improve the lipid accumulation of diatom cells, Nitzschia sp. was mutated by Co-60-gamma-ray irradiation and cultivated with high salinity. The biomass and lipid yields of Nitzschia sp. mutated by Co-60-gamma-ray irradiation at 900 Gy were increased by 53.8% and 28.1%, respectively. The lipid content of Nitzschia sp. mutant cells cultivated with gradually increased salinity of up to 30% was increased from 11.9% to 27.2%. This was because that the increased demand for nitrogen to keep cells healthy growth under high salinity conditions resulted in lipid accumulation, which was beneficial to prevent cell membranes from being destroyed by changing osmotic pressure. When the Nitzschia-ZJU1 strain obtained from Nitzschia sp. by irradiation mutation and salinity domestication was cultured in the growth media with no nitrogen and no silicon, the lipid content of cells was dramatically increased to 51.2%. (C) 2014 Elsevier Ltd. All rights reserved.
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