Journal
ENDOCRINOLOGY
Volume 155, Issue 10, Pages 3757-3768Publisher
ENDOCRINE SOC
DOI: 10.1210/en.2013-2051
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Funding
- National Institutes of Health [DK096122, DK081666]
- Pilot and Feasibility grant through Vanderbilt University Diabetes Research Training Center [P60 DK20593]
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Calcium entry through voltage-dependent Ca2+ channels (VDCCs) is required for pancreatic beta-cell insulin secretion. The 2-pore-domain acid-sensitive potassium channel (TASK-1) regulates neuronal excitability and VDCC activation by hyperpolarizing the plasma membrane potential (Delta Psi p); however, a role for pancreatic beta-cell TASK-1 channels is unknown. Here we examined the influence of TASK-1 channel activity on the beta-cell Delta Psi p and insulin secretion during secretagogue stimulation. TASK-1 channels were found to be highly expressed in human and rodent islets and localized to the plasma membrane of beta-cells. TASK-1-like currents of mouse and human beta-cells were blocked by the potent TASK-1 channel inhibitor, A1899 (250nM). Although inhibition of TASK-1 currents did not influence the beta-cell Delta Psi p in the presence of low (2mM) glucose, A1899 significantly enhanced glucose-stimulated(14mM) Delta Psi p depolarization of human and mouse beta-cells. TASK-1 inhibition also resulted in greater secretagogue-stimulated Ca2+ influx in both human and mouse islets. Moreover, conditional ablation of mouse beta-cell TASK-1 channels reduced K2P currents, increased glucose-stimulated Delta Psi p depolarization, and augmented secretagogue-stimulated Ca2+ influx. The Delta Psi p depolarization caused by TASK-1 inhibition resulted in a transient increase in glucose-stimulated mouse beta-cell action potential (AP) firing frequency. However, secretagogue-stimulated beta-cell AP duration eventually increased in the presence of A1899 as well as in beta-cells without TASK-1, causing a decrease in AP firing frequency. Ablation or inhibition of mouse beta-cell TASK-1 channels also significantly enhanced glucose-stimulated insulin secretion, which improved glucose tolerance. Conversely, TASK-1 ablation did not perturb beta-cell Delta Psi p, Ca2+ influx, or insulin secretion under low-glucose conditions (2mM). These results reveal a glucose-dependent role for beta-cell TASK-1 channels of limiting glucose-stimulated Delta Psi p depolarization and insulin secretion, which modulates glucose homeostasis.
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