Journal
ENDOCRINOLOGY
Volume 150, Issue 12, Pages 5334-5340Publisher
ENDOCRINE SOC
DOI: 10.1210/en.2009-0600
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Funding
- Swedish Research Council
- Swedish Diabetes Association
- Novo Nordic Fund
- Family Ernfors Fund
- Albert Pahlsson Foundation
- Crafoord Foundation
- Juvenile Diabetes Research Foundation
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The kinetics of insulin, glucagon and somatostatin release was studied in human pancreatic islets. Batches of 10-15 islets were perifused and the hormones measured with RIA in 30-sec fractions. Increase of glucose from 3 to 20 mM resulted in a brief pulse of glucagon coinciding with suppression of basal insulin and somatostatin release. There was a subsequent drop of glucagon release concomitant with the appearance of a pronounced pulse of insulin and a slightly delayed pulse of somatostatin. Continued exposure to 20 mM glucose generated pulsatile release of the three hormones with 7- to 8-min periods accounting for 60-70% of the secreted amounts. Glucose caused pronounced stimulation of average insulin and somatostatin release. However, the nadirs between the glucagon pulses were lower than the secretion at 3 mM glucose, resulting in 18% suppression of average release. The repetitive glucagon pulses were antisynchronous to coincident pulses of insulin and somatostatin. The resulting greater than 20-fold variations of the insulin to glucagon ratio might be essential for minute-to-minute regulation of the hepatic glucose production. (Endocrinology 150: 5334-5340, 2009)
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