Journal
JOURNAL OF PLANT PHYSIOLOGY
Volume 174, Issue -, Pages 5-15Publisher
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2014.08.022
Keywords
Salinity; Antioxidant defense system; Atrboh; H2O2; Arabidopsis thaliana
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Funding
- Tunisian Ministry of Higher Education and Scientific Research [LR10CBBC02]
- Tunisian French CMCU (Comite Mixte de Cooperation Universitaire) network [13G0929]
- European Union COST program FA0901 Putting halophytes to work - From genesto ecosystem
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The involvement of hydrogen peroxide (H2O2) generated by nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase) in the antioxidant defense system was assessed in salt-challenged Arabidopsis thaliana seedlings. In the wild-type, short-term salt exposure led to a transient and significant increase of H2O2 concentration, followed by a marked increase in catalase (CAT, EC 1.11.16), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) activities. Pre-treatment with either a chemical trap for H2O2 (dimethylthiourea) or two widely used NADPH oxidase inhibitors (imidazol and diphenylene iodonium) significantly decreased the above-mentioned enzyme activities under salinity. Double mutant atrbohd/f plants failed to induce the antioxidant response under the culture conditions. Under long-term salinity, the wild-type was more salt-tolerant than the mutant based on the plant biomass production. The better performance of the wild-type was related to a significantly higher photosynthetic activity, a more efficient K+ selective uptake, and to the plants' ability to deal with the salt-induced oxidative stress as compared to atrbohd/f. Altogether, these data suggest that the early H2O2 generation by NADPH oxidase under salt stress could be the beginning of a reaction cascade that triggers the antioxidant response in A. thaliana in order to overcome the subsequent reactive oxygen species (ROS) production, thereby mitigating the salt stress-derived injuries. (C) 2014 Elsevier GmbH. All rights reserved.
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