Journal
ENDOCRINE JOURNAL
Volume 58, Issue 7, Pages 575-583Publisher
JAPAN ENDOCRINE SOC
DOI: 10.1507/endocrj.K11E-051
Keywords
Cerenkov radiation; Radioiodine; Optical imaging; Thyroid gland; NIS
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Funding
- Ministry of Education, Science & Technology (MEST)
- National Research Foundation of Korea (NRF) [001-7540]
- Ministry of Education, Science & Technology (MEST) [2010-0017514]
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Radioiodine (RI) such as (131)I or (124)I, can generate luminescent emission and be detected with an optical imaging (01) device. To evaluate the possibility of a novel Cerenkov luminescence imaging (CLI) for application in thyroid research, we performed feasibility studies of CLI by RI in the thyroid gland and human anaplastic thyroid carcinoma cells expressing sodium iodide symporter gene (ARO-NIS). For in vitro study, FRTL-5 and ARO-NIS were incubated with RI, and the luminometric and CLI intensity was measured with luminometer and 01 device. Luminescence intensity was compared with the radioactivity measured with gamma-counter. In vivo CLI of the thyroid gland was performed in mice after intravenous injection of RI with and without thyroid blocking. Mice were implanted with ARO-NIS subcutaneously, and CLI was performed with injection of (124)I. Small animal PET or gamma-camera imaging was also performed. CLI intensities of thyroid gland and ARO-NIS were quantified, and compared with the radioactivities measured from nuclear images (NI). Luminometric assay and OI confirmed RI uptake in the cells in a dose-dependent manner, and luminescence intensity was well correlated with radioactivity of the cells. CLI clearly demonstrated RI uptake in thyroid gland and xenografted ARO-NIS cells in mice, which was further confirmed by NI. A strong positive correlation was observed between CLI intensity and radioactivity assessed by NI. We successfully demonstrated dual molecular imaging of CLI and NI using RI both in vitro and in vivo. CLI can provide a new OI strategy in preclinical thyroid studies.
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