Journal
EMBO REPORTS
Volume 14, Issue 5, Pages 434-440Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/embor.2013.36
Keywords
membrane contact sites; phosphatidylcholine synthesis; Opi3; Osh3; Pah1
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Funding
- Canadian Institutes of Health Research
- Michael Smith Foundation for Health Research
- Canadian Foundation for Innovation
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Synthesis of phospholipids, sterols and sphingolipids is thought to occur at contact sites between the endoplasmic reticulum (ER) and other organelles because many lipid-synthesizing enzymes are enriched in these contacts. In only a few cases have the enzymes been localized to contacts in vivo and in no instances have the contacts been demonstrated to be required for enzyme function. Here, we show that plasma membrane (PM)-ER contact sites in yeast are required for phosphatidylcholine synthesis and regulate the activity of the phosphatidylethanolamine N-methyltransferase enzyme, Opi3. Opi3 activity requires Osh3, which localizes to PM-ER contacts where it might facilitate in trans catalysis by Opi3. Thus, membrane contact sites provide a structural mechanism to regulate lipid synthesis.
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