Journal
EMBO REPORTS
Volume 14, Issue 4, Pages 364-372Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/embor.2013.23
Keywords
Beclin-1; chromosome congression; kinetochore assembly; mitosis; Zwint-1
Categories
Funding
- Ministere Francais de l'enseignement superieur et de la Recherche
- Fondation pour la Recherche Medicale
- SIDACTION
- ANRS
- [ANR-07-JCJC-0102]
Ask authors/readers for more resources
The functions of Beclin-1 in macroautophagy, tumorigenesis and cytokinesis are thought to be mediated by its association with the PI3K-III complex. Here, we describe a new role for Beclin-1 in mitotic chromosome congression that is independent of the PI3K-III complex and its role in autophagy. Beclin-1 depletion in HeLa cells leads to a significant reduction of the outer kinetochore proteins CENP-E, CENP-F and ZW10, and, consequently, the cells present severe problems in chromosome congression. Beclin-1 associates with kinetochore microtubules and forms discrete foci near the kinetochores of attached chromosomes. We show that Beclin-1 interacts directly with Zwint-1-a component of the KMN (KNL-1/Mis12/Ndc80) complex-which is essential for kinetochore-microtubule interactions. This suggests that Beclin-1 acts downstream of the KMN complex to influence the recruitment of outer kinetochore proteins and promotes accurate kinetochore anchoring to the spindle during mitosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available