Journal
EMBO REPORTS
Volume 14, Issue 6, Pages 545-552Publisher
WILEY
DOI: 10.1038/embor.2013.56
Keywords
glutathione S-transferase; mitochondrial dynamics; peripheral neuropathy; tail-anchored protein
Categories
Funding
- Swiss National Science Foundation
- National Center for Competence in Research (NCCR) Neural Plasticity and Repair
- BBSRC [BB/K006231/1]
- Portuguese Foundation for Science and Technology (FCT)
- Fundo Europeu De Desenvolvimento Regional (FEDER) [PTDC/SAU-OSM/103647/2008, PTDC/BIA-BCM/118605/2010, SFRH/BD/73532/2010]
- BBSRC [BB/K006231/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/K006231/1] Funding Source: researchfish
- Fundação para a Ciência e a Tecnologia [PTDC/BIA-BCM/118605/2010, PTDC/SAU-OSM/103647/2008, SFRH/BD/73532/2010] Funding Source: FCT
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Mitochondria and peroxisomes can be fragmented by the process of fission. The fission machineries of both organelles share a set of proteins. GDAP1 is a tail-anchored protein of mitochondria and induces mitochondrial fragmentation. Mutations in GDAP1 lead to Charcot-Marie-Tooth disease (CMT), an inherited peripheral neuropathy, and affect mitochondrial dynamics. Here, we show that GDAP1 is also targeted to peroxisomes mediated by the import receptor Pex19. Knockdown of GDAP1 leads to peroxisomal elongation that can be rescued by re-expressing GDAP1 and by missense mutated forms found in CMT patients. GDAP1-induced peroxisomal fission is dependent on the integrity of its hydrophobic domain 1, and on Drp1 and Mff, as is mitochondrial fission. Thus, GDAP1 regulates mitochondrial and peroxisomal fission by a similar mechanism. However, our results reveal also a more critical role of the amino-terminal GDAP1 domains, carrying most CMT-causing mutations, in the regulation of mitochondrial compared to peroxisomal fission.
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