Journal
EMBO REPORTS
Volume 13, Issue 3, Pages 237-243Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/embor.2011.249
Keywords
cell division axis; FRET; polarity; Rho family GTPases; tight junction
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Japan Society for the Promotion of Science for Young Scientists
- Grants-in-Aid for Scientific Research [22113002, 10J05775, 22113001, 22590358] Funding Source: KAKEN
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Using MDCK cells that constitutively express a Forster resonance energy transfer biosensor, we found that Rac1 activity is homogenous at the entire plasma membrane in early stages of cystogenesis, whereas in later stages Rac1 activity is higher at the lateral membrane than at the apical plasma membrane. If Rac1 is activated at the apical membrane in later stages, however, the monolayer cells move into the luminal space. In these cells, tight junctions are disrupted, accompanied by mislocalization of polarization markers and disorientation of cell division. These observations indicate that Rac1 suppression at the apical membrane is essential for the maintenance of cyst structure.
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