Journal
EMBO REPORTS
Volume 13, Issue 11, Pages 997-1003Publisher
WILEY
DOI: 10.1038/embor.2012.146
Keywords
Chd1; chromatin remodelers; cryptic transcription; nucleosome structure; S.pombe
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Funding
- Ministry of Science, Research
- Arts of Baden-Wuerttemberg
- China Scholarship Council (CSC)
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Chromatin organization is essential for defining transcription units and maintaining genomic integrity in eukaryotes. In this study, we found that deletion of the Schizosaccharomyces pombe Chd1 chromatin remodelers, hrp1 and hrp3, causes strong, genome-wide accumulation of antisense transcripts. Nucleosome mapping revealed a specific role for Chd1 remodelers in the positioning of nucleosomes in gene coding regions. Other mutations associated with enhanced cryptic transcription activity, such as set2 Delta, alp13 Delta and FACT complex subunit pob3 Delta, did not, or only mildly, affect nucleosome positioning. These data indicate several mechanisms in the repression of cryptic promoter activity in eukaryotic cells.
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