Journal
EMBO REPORTS
Volume 12, Issue 9, Pages 944-949Publisher
WILEY
DOI: 10.1038/embor.2011.128
Keywords
Argonaute; microRNA; RISC
Categories
Funding
- Japan Ministry of Education, Culture, Sports, Science and Technology
- The International Human Frontier Science Program Organization
- Grants-in-Aid for Scientific Research [10J06426, 22687014] Funding Source: KAKEN
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MicroRNAs (miRNAs) function through the RNA-induced silencing complex (RISC), which contains an Argonaute (Ago) protein at the core. RISC assembly follows a two-step pathway: miRNA/miRNA* duplex loading into Ago, and separation of the two strands within Ago. Here we show that the 5' phosphate of the miRNA strand is essential for duplex loading into Ago, whereas the preferred 5' nucleotide of the miRNA strand and the base-pairing status in the seed region and the middle of the 3' region function as additive anchors to Ago. Consequently, the miRNA authenticity is inspected at multiple steps during RISC assembly.
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