Journal
EMBO REPORTS
Volume 11, Issue 4, Pages 292-298Publisher
WILEY
DOI: 10.1038/embor.2010.10
Keywords
focal adhesions; microtubules; Rac; STEF; Tiam2
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Funding
- Bioimaging Facility of the Faculty of Life Sciences (The University of Manchester)
- Biotechnology and Biological Sciences Research Council [BB/G004552/1]
- Cancer Research UK [C147/A6058]
- BBSRC [BB/G004552/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G004552/1] Funding Source: researchfish
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Focal adhesion (FA) disassembly required for optimal cell migration is mediated by microtubules (MTs); targeting of FAs by MTs coincides with their disassembly. Regrowth of MTs, induced by removal of the MT destabilizer nocodazole, activates the Rho-like GTPase Rac, concomitant with FA disassembly. Here, we show that the Rac guanine nucleotide exchange factor (GEF) Sif and Tiam1-like exchange factor (STEF) is responsible for Rac activation during MT regrowth. Importantly, STEF is required for multiple targeting of FAs by MTs. As a result, FAs in STEF-knockdown cells have a reduced disassembly rate and are consequently enlarged. This leads to reduced speed of migration. Together, these findings suggest a new role for STEF in FA disassembly and cell migration through MT-mediated mechanisms.
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