Journal
EMBO REPORTS
Volume 11, Issue 6, Pages 445-451Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/embor.2010.64
Keywords
alternative pre-mRNA splicing; FLIM-FRET; CDC5L; hnRNP-M; spliceosome
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Funding
- Wellcome Trust
- European Alternative Splicing Network (Eurasnet)
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Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 50 and 30 alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.
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