Journal
EMBO REPORTS
Volume 10, Issue 2, Pages 144-151Publisher
WILEY
DOI: 10.1038/embor.2008.256
Keywords
Argonaute; integrase; protein structure; RNase H; transposase
Categories
Funding
- European Molecular Biology Organization
- Wellcome Trust Senior Research Fellowship
Ask authors/readers for more resources
The retroviral integrase superfamily (RISF) comprises numerous important nucleic acid-processing enzymes, including transposases, integrases and various nucleases. These enzymes are involved in a wide range of processes such as transposition, replication and repair of DNA, homologous recombination, and RNA-mediated gene silencing. Two out of the four enzymes that are encoded by the human immunodeficiency virus-RNase H1 and integrase-are members of this superfamily. RISF enzymes act on various substrates, and yet show remarkable mechanistic and structural similarities. All share a common fold of the catalytic core and the active site, which is composed primarily of carboxylate residues. Here, I present RISF proteins from a structural perspective, describing the individual members and the common and divergent elements of their structures, as well as the mechanistic insights gained from the structures of RNase H1 enzyme complexes with RNA/DNA hybrids.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available