Journal
EMBO JOURNAL
Volume 33, Issue 18, Pages 2080-2097Publisher
WILEY-BLACKWELL
DOI: 10.15252/embj.201488367
Keywords
live cell imaging; Rab6; retrograde transport; secretory pathway; VSV glycoprotein
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Funding
- Italian Association for Cancer Research (AIRC)
- CNR Research Project on Aging
- Regione Lombardia Project MbMM-convenzione [18099/RCC]
- University of Milan
- Fondazione Confalonieri (Milan)
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The Golgi complex and ER are dynamically connected by anterograde and retrograde trafficking pathways. To what extent and by what mechanism outward-bound cargo proteins escape retrograde trafficking has been poorly investigated. Here, we analysed the behaviour of several membrane proteins at the ER/Golgi interface in live cells. When Golgi-to-plasma membrane transport was blocked, vesicular stomatitis virus glycoprotein (VSVG), which bears an ER export signal, accumulated in the Golgi, whereas an export signal-deleted version of VSVG attained a steady state determined by the balance of retrograde and anterograde traffic. A similar behaviour was displayed by EGF receptor and by a model tail-anchored protein, whose retrograde traffic was slowed by addition of VSVG's export signal. Retrograde trafficking was energy- and Rab6-dependent, and Rab6 inhibition accelerated signal-deleted VSVG's transport to the cell surface. Our results extend the dynamic bi-directional relationship between the Golgi and ER to include surface-directed proteins, uncover an unanticipated role for export signals at the Golgi complex, and identify recycling as a novel factor that regulates cargo transport out of the early secretory pathway.
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