Journal
EMBO JOURNAL
Volume 32, Issue 10, Pages 1352-1364Publisher
WILEY
DOI: 10.1038/emboj.2013.59
Keywords
axonal transport; CFEOM3; kinesin; TUBB2; TUBB3
Categories
Funding
- Carl Zeiss
- JEOL
- Global COE program from Ministry of Education, Culture, Sports, Science and Technology of Japan
Ask authors/readers for more resources
Microtubules are fundamental to neuronal morphogenesis and function. Mutations in tubulin, the major constituent of microtubules, result in neuronal diseases. Here, we have analysed beta-tubulin mutations that cause neuronal diseases and we have identified mutations that strongly inhibit axonal transport of vesicles and mitochondria. These mutations are in the H12 helix of beta-tubulin and change the negative charge on the surface of the microtubule. This surface is the interface between microtubules and kinesin superfamily motor proteins (KIF). The binding of axonal transport KIFs to microtubules is dominant negatively disrupted by these mutations, which alters the localization of KIFs in neurons and inhibits axon elongation in vivo. In humans, these mutations induce broad neurological symptoms, such as loss of axons in the central nervous system and peripheral neuropathy. Thus, our data identified the critical region of beta-tubulin required for axonal transport and suggest a molecular mechanism for human neuronal diseases caused by tubulin mutations.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available