Journal
EMBO JOURNAL
Volume 31, Issue 13, Pages 2994-3007Publisher
WILEY
DOI: 10.1038/emboj.2012.136
Keywords
chromatin; differentiation; histone variant; myogenesis
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Kaibara Morikazu Medical Science Promotion Foundation
- NIH [GM56244]
- Grants-in-Aid for Scientific Research [23651195, 23310134, 22390292, 20114001, 23650327] Funding Source: KAKEN
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Cell differentiation is mediated by lineage-determining transcription factors. We show that chromodomain helicase DNA-binding domain 2 (Chd2), a SNF2 chromatin remodelling enzyme family member, interacts with MyoD and myogenic gene regulatory sequences to specifically mark these loci via deposition of the histone variant H3.3 prior to cell differentiation. Directed and genome-wide analysis of endogenous H3.3 incorporation demonstrates that knockdown of Chd2 prevents H3.3 deposition at differentiation-dependent, but not housekeeping, genes and inhibits myogenic gene activation. The data indicate that MyoD determines cell fate and facilitates differentiation-dependent gene expression through Chd2-dependent deposition of H3.3 at myogenic loci prior to differentiation. The EMBO Journal (2012) 31, 2994-3007. doi: 10.1038/emboj.2012.136; Published online 8 May 2012
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