Journal
EMBO JOURNAL
Volume 32, Issue 1, Pages 30-44Publisher
WILEY
DOI: 10.1038/emboj.2012.305
Keywords
apical polarisation; lumen formation; Pard3; Rablla; zebrafish
Categories
Funding
- BBSRC
- MRC
- Wellcome Trust
- National Institute of Health
- National Eye Institute
- BBSRC [BB/H00260X/1] Funding Source: UKRI
- MRC [G0901899] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H00260X/1] Funding Source: researchfish
- Medical Research Council [G0901899] Funding Source: researchfish
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By analysing the cellular and subcellular events that occur in the centre of the developing zebrafish neural rod, we have uncovered a novel mechanism of cell polarisation during lumen formation. Cells from each side of the neural rod interdigitate across the tissue midline. This is necessary for localisation of apical junctional proteins to the region where cells intersect the tissue midline. Cells assemble a mirror-symmetric microtubule cytoskeleton around the tissue midline, which is necessary for the trafficking of proteins required for normal lumen forma:ton, such as partitioning defective 3 and Rablla to this point. This occurs in advance and is independent of the midline cell division that has been shown to have a powerful role in lumen organisation. To our knowledge, this is the first example of the initiation of apical polarisation part way along the length of a cell, rather than at a cell extremity. Although the midline division is not necessary for apical polarisation, it confers a morphogenetic advantage by efficiently eliminating cellular processes that would otherwise bridge the developing lumen. The EMBO Journal (2013) 32, 30-44. doi:10.1038/emboj.2012.305; Published online 30 November 2012
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