Journal
EMBO JOURNAL
Volume 30, Issue 16, Pages 3309-3321Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/emboj.2011.226
Keywords
centromere; chromatin remodelling; DNA repair; mitosis
Categories
Funding
- National Institutes of Health [GM61542, GM76481, GM090077]
- Welch Foundation [I-1441, I-1713]
- March of Dimes Foundation
- American Heart Association
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Centromeres nucleate the formation of kinetochores and are vital for chromosome segregation during mitosis. The SNF2 family helicase PICH (Plk1-interacting checkpoint helicase) and the BLM (the Bloom's syndrome protein) helicase decorate ultrafine histone-negative DNA threads that link the segregating sister centromeres during anaphase. The functions of PICH and BLM at these threads are not understood, however. Here, we show that PICH binds to BLM and enables BLM localization to anaphase centromeric threads. PICH- or BLM-RNAi cells fail to resolve these threads in anaphase. The fragmented threads form centromeric-chromatin-containing micronuclei in daughter cells. Anaphase threads in PICH- and BLM-RNAi cells contain histones and centromere markers. Recombinant purified PICH has nucleosome remodelling activities in vitro. We propose that PICH and BLM unravel centromeric chromatin and keep anaphase DNA threads mostly free of nucleosomes, thus allowing these threads to span long distances between rapidly segregating centromeres without breakage and providing a spatiotemporal window for their resolution. The EMBO Journal (2011) 30, 3309-3321. doi:10.1038/emboj.2011.226; Published online 8 July 2011
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