Journal
EMBO JOURNAL
Volume 30, Issue 13, Pages 2569-2581Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/emboj.2011.151
Keywords
AP-2 gamma; chromatin immunoprecipitation and sequencing; FoxA1; long-range chromatin interaction; oestrogen receptor
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Funding
- Biomedical Research Council/Science and Engineering Research Council of A*STAR (Agency for Science, Technology and Research), Singapore
- NUS
- NUS Graduate School (NGS) for Integrative Sciences and Engineering
- A*STAR
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Oestrogen receptor a (ER alpha) is key player in the progression of breast cancer. Recently, the cistrome and interactome of ER alpha were mapped in breast cancer cells, revealing the importance of spatial organization in oestrogen-mediated transcription. However, the underlying mechanism of this process is unclear. Here, we show that ER alpha binding sites (ERBS) identified from the Chromatin Interaction Analysis-Paired End DiTag of ER alpha are enriched for AP-2 motifs. We demonstrate the transcription factor, AP-2 gamma, which has been implicated in breast cancer oncogenesis, binds to ERBS in a ligand-independent manner. Furthermore, perturbation of AP-2 gamma expression impaired ER alpha DNA binding, long-range chromatin interactions, and gene transcription. In genome-wide analyses, we show that a large number of AP-2 gamma and ER alpha binding events converge together across the genome. The majority of these shared regions are also occupied by the pioneer factor, FoxA1. Molecular studies indicate there is functional interplay between AP-2 gamma and FoxA1. Finally, we show that most ERBS associated with long-range chromatin interactions are colocalized with AP-2 gamma and FoxA1. Together, our results suggest AP-2 gamma is a novel collaborative factor in ER alpha-mediated transcription. The EMBO Journal (2011) 30, 2569-2581. doi:10.1038/emboj.2011.151; Published online 13 May 2011
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