Journal
EMBO JOURNAL
Volume 30, Issue 21, Pages 4371-4386Publisher
WILEY
DOI: 10.1038/emboj.2011.365
Keywords
cAMP; cell signalling; lipolysis; OPA1; perilipin
Categories
Funding
- Norwegian Functional Genomics Program
- Research Council of Norway
- Novo Nordic Foundation
- European Union [037189]
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Adrenergic stimulation of adipocytes yields a cAMP signal that activates protein kinase A (PKA). PKA phosphorylates perilipin, a protein localized on the surface of lipid droplets that serves as a gatekeeper to regulate access of lipases converting stored triglycerides to free fatty acids and glycerol in a phosphorylation-dependent manner. Here, we report a new function for optic atrophy 1 (OPA1), a protein known to regulate mitochondrial dynamics, as a dual-specificity A-kinase anchoring protein associated with lipid droplets. By a variety of protein interaction assays, immunoprecipitation and immunolocalization experiments, we show that OPA1 organizes a supramolecular complex containing both PKA and perilipin. Furthermore, by a combination of siRNA-mediated knockdown, reconstitution experiments using full-length OPA1 with or without the ability to bind PKA or truncated OPA1 fused to a lipid droplet targeting domain and cellular delivery of PKA anchoring disruptor peptides, we demonstrate that OPA1 targeting of PKA to lipid droplets is necessary for hormonal control of perilipin phosphorylation and lipolysis. The EMBO Journal (2011) 30, 4371-4386. doi:10.1038/emboj.2011.365; Published online 7 October 2011
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