Journal
EMBO JOURNAL
Volume 29, Issue 8, Pages 1377-1388Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/emboj.2010.30
Keywords
angiogenic sprouting; embryoid body; heterodimer; proximity ligation; VEGF receptor
Categories
Funding
- Swedish Cancer Foundation
- Swedish Research Council
- EU [LSHG.CT2004-503573]
- Wallenberg Foundation
- Swedish Foundation for Strategic Research
Ask authors/readers for more resources
The vascular endothelial growth factors VEGFA and VEGFC are crucial regulators of vascular development. They exert their effects by dimerization and activation of the cognate receptors VEGFR2 and VEGFR3. Here, we have used in situ proximity ligation to detect receptor complexes in intact endothelial cells. We show that both VEGFA and VEGFC potently induce formation of VEGFR2/-3 heterodimers. Receptor heterodimers were found in both developing blood vessels and immature lymphatic structures in embryoid bodies. We present evidence that heterodimers frequently localize to tip cell filopodia. Interestingly, in the presence of VEGFC, heterodimers were enriched in the leading tip cells as compared with trailing stalk cells of growing sprouts. Neutralization of VEGFR3 to prevent heterodimer formation in response to VEGFA decreased the extent of angiogenic sprouting. We conclude that VEGFR2/-3 heterodimers on angiogenic sprouts induced by VEGFA or VEGFC may serve to positively regulate angiogenic sprouting. The EMBO Journal (2010) 29, 1377-1388. doi: 10.1038/emboj.2010.30; Published online 11 March 2010
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available