Journal
EMBO JOURNAL
Volume 29, Issue 18, Pages 3068-3081Publisher
WILEY
DOI: 10.1038/emboj.2010.207
Keywords
Caulobacter; chromosome segregation; ParA; TipN
Categories
Funding
- National Institutes of Health [GM065835]
- Yale University
- [1F31GM083627]
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What regulates chromosome segregation dynamics in bacteria is largely unknown. Here, we show in Caulobacter crescentus that the polarity factor TipN regulates the directional motion and overall translocation speed of the parS/ParB partition complex by interacting with ParA at the new pole. In the absence of TipN, ParA structures can regenerate behind the partition complex, leading to stalls and back-and-forth motions of parS/ParB, reminiscent of plasmid behaviour. This extrinsic regulation of the parS/ParB/ParA system directly affects not only division site selection, but also cell growth. Other mechanisms, including the pole-organizing protein PopZ, compensate for the defect in segregation regulation in Lambda tipN cells. Accordingly, synthetic lethality of PopZ and TipN is caused by severe chromosome segregation and cell division defects. Our data suggest a mechanistic framework for adapting a self-organizing oscillator to create motion suitable for chromosome segregation.
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