Journal
EMBO JOURNAL
Volume 29, Issue 13, Pages 2126-2134Publisher
WILEY
DOI: 10.1038/emboj.2010.109
Keywords
chromatin; gene expression; remodelling; topoisomerase
Categories
Funding
- Swedish Cancer Society
- Swedish Research Council
- EU 'The Epigenome' NoE network
- Cancer Research UK [11809] Funding Source: researchfish
Ask authors/readers for more resources
DNA topoisomerases regulate the topological state of the DNA double helix and are key enzymes in the processes of DNA replication, transcription and genome stability. Using the fission yeast model Schizosaccharomyces pombe, we investigate genome wide how DNA topoisomerases I and II affect chromatin dynamics and gene expression in vivo. We show that topoisomerase I activity is directly required for efficient nucleosome disassembly at gene promoter regions. Lack of topoisomerase activity results in increased nucleosome occupancy, perturbed histone modifications and reduced transcription from these promoters. Strong correlative evidence suggests that topoisomerase I cooperates with the ATP-dependent chromatin remodeller Hrp1 in nucleosome disassembly. Our study links topoisomerase activity to the maintenance of open chromatin and regulating transcription in vivo. The EMBO Journal (2010) 29, 2126-2134. doi:10.1038/emboj.2010.109; Published online 4 June 2010
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available