Journal
EMBO JOURNAL
Volume 29, Issue 20, Pages 3470-3483Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/emboj.2010.221
Keywords
dishevelled; microtubule plus end; mitosis; Plk1; Wnt signalling
Categories
Funding
- Ministry of Education, Science, and Culture of Japan
- Princess Takamatsu Cancer Research Fund [08-24005]
- Takeda Science Foundation
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Wnt signalling is known to promote G1/S progression through the stimulation of gene expression, but whether this signalling regulates mitotic progression is not clear. Here, the function of dishevelled 2 (Dvl2), which transmits the Wnt signal, in mitosis was examined. Dvl2 localized to the spindles and spindle poles during mitosis. When cells were treated with nocodazole, Dvl2 was observed at the kinetochores (KTs). Dvl2 bound to and was phosphorylated at Thr206 by a mitotic kinase, Polo-like kinase 1 (Plk1), and this phosphorylation was required for spindle orientation and stable microtubule (MT)-KT attachment. Dvl2 was also found to be involved in the activation of a spindle assembly checkpoint (SAC) kinase, Mps1, and the recruitment of other SAC components, Bub1 and BubR1, to the KTs. However, the phosphorylation of Dvl2 by Plk1 was dispensable for SAC. Furthermore, Wnt receptors were involved in spindle orientation, but not in MT-KT attachment or SAC. These results suggested. that Dvl2 is involved in mitotic progression by regulating the dynamics of MT plus-ends and the SAC in Plk1-dependent and -independent manners. The EMBO Journal (2010) 29, 3470-3483. doi:10.1038/emboj.2010.221; Published online 7 September 2010 Subject Categories: signal transduction; cell cycle
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