Journal
EMBO JOURNAL
Volume 28, Issue 21, Pages 3428-3438Publisher
WILEY
DOI: 10.1038/emboj.2009.262
Keywords
EFR; ER-quality control; pathogen-associated molecular patterns; pattern-recognition receptor; SDF2
Categories
Funding
- Biotechnology and Biological Sciences Research Council [BB/F021046/1]
- ERA-NET Plant Genomics
- Gatsby Charitable Foundation
- Swiss National Foundation [31003A-120655]
- BBSRC [BB/F021046/1, BB/E024874/1] Funding Source: UKRI
- Swiss National Science Foundation (SNF) [31003A-120655] Funding Source: Swiss National Science Foundation (SNF)
- Biotechnology and Biological Sciences Research Council [BB/F021046/1, BB/E024874/1] Funding Source: researchfish
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In plant innate immunity, the surface-exposed leucine-rich repeat receptor kinases EFR and FLS2 mediate recognition of the bacterial pathogen-associated molecular patterns EF-Tu and flagellin, respectively. We identified the Arabidopsis stromal-derived factor-2 (SDF2) as being required for EFR function, and to a lesser extent FLS2 function. SDF2 resides in an endoplasmic reticulum (ER) protein complex with the Hsp40 ERdj3B and the Hsp70 BiP, which are components of the ER-quality control (ER-QC). Loss of SDF2 results in ER retention and degradation of EFR. The differential requirement for ER-QC components by EFR and FLS2 could be linked to N-glycosylation mediated by STT3a, a catalytic subunit of the oligosaccharyltransferase complex involved in co-translational N-glycosylation. Our results show that the plasma membrane EFR requires the ER complex SDF2-ERdj3B-BiP for its proper accumulation, and provide a demonstration of a physiological requirement for ER-QC in transmembrane receptor function in plants. They also provide an unexpected differential requirement for ER-QC and N-glycosylation components by two closely related receptors. The EMBO Journal (2009) 28, 3428-3438. doi: 10.1038/emboj.2009.262; Published online 17 September 2009
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