Journal
EMBO JOURNAL
Volume 28, Issue 5, Pages 513-522Publisher
WILEY
DOI: 10.1038/emboj.2008.285
Keywords
A20; Itch; NF-kappa B; RNF11; TAX1BP1
Categories
Funding
- Department of Microbiology and Immunology
- University of Miami Miller School of Medicine
- Sylvester Comprehensive Cancer Center
- American Cancer Society
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The RING domain protein RNF11 is overexpressed in breast cancers and promotes tumour growth factor-beta (TGF-beta) signalling. RNF11 has been proposed to regulate TGF-beta signalling by interacting with HECT- and SCF-type E3 ligases; however, the role of RNF11 in other signalling pathways is poorly understood. Here, we demonstrate a novel function of RNF11 as a negative regulator of NF-kappa B and jun N-terminal kinase (JNK) signalling pathways. Knockdown of RNF11 with siRNA resulted in persistent tumour necrosis factor (TNF)- and lipopolysaccharide (LPS)-mediated NF-kappa B and JNK signalling. RNF11 interacted with the NF-kappa B inhibitor A20 and its regulatory protein TAX1BP1 in a stimulus-dependent manner. RNF11 negatively regulated RIP1 and TRAF6 ubiquitination upon stimulation with TNF and LPS, respectively. Furthermore, RNF11 was required for A20 to interact with and inactivate RIP1 to inhibit TNF-mediated NF-kappa B activation. Our studies reveal that RNF11, together with TAX1BP1 and Itch, is an essential component of an A20 ubiquitin-editing protein complex that ensures transient activation of inflammatory signalling pathways.
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