Journal
EMBO JOURNAL
Volume 28, Issue 20, Pages 3185-3195Publisher
WILEY
DOI: 10.1038/emboj.2009.258
Keywords
apoptosis; DNA damage; E2F1; HCF-1; MLL
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Funding
- Swiss National Science Foundation, Oncosuisse
- University of Lausanne
- EMBO long-term fellowship
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E2F1 is a key positive regulator of human cell proliferation and its activity is altered in essentially all human cancers. Deregulation of E2F1 leads to oncogenic DNA damage and anti-oncogenic apoptosis. The molecular mechanisms by which E2F1 mediates these two processes are poorly understood but are important for understanding cancer progression. During the G1-to-S phase transition, E2F1 associates through a short DHQY sequence with the cell-cycle regulator HCF-1 together with the mixed-lineage leukaemia (MLL) family of histone H3 lysine 4 (H3K4) methyltransferases. We show here that the DHQY HCF-1-binding sequence permits E2F1 to stimulate both DNA damage and apoptosis, and that HCF-1 and the MLL family of H3K4 methyltransferases have important functions in these processes. Thus, HCF-1 has a broader role in E2F1 function than appreciated earlier. Indeed, sequence changes in the E2F1 HCF-1-binding site can modulate both up and down the ability of E2F1 to induce apoptosis indicating that HCF-1 association with E2F1 is a regulator of E2F1-induced apoptosis. The EMBO Journal (2009) 28, 3185-3195. doi:10.1038/emboj.2009.258; Published online 17 September 2009
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