Journal
EMBO JOURNAL
Volume 29, Issue 1, Pages 80-92Publisher
WILEY
DOI: 10.1038/emboj.2009.307
Keywords
no go decay; ribosome biogenesis; Rio1p; translation initiation; yeast
Categories
Funding
- CNRS
- Universite Paul Sabatier
- La Ligue Nationale contre le Cancer (Equipe Labellisee)
- Agence Nationale de la Recherche
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It is generally assumed that, in Saccharomyces cerevisiae, immature 40S ribosomal subunits are not competent for translation initiation. Here, we show by different approaches that, in wild-type conditions, a portion of pre-40S particles (pre-SSU) associate with translating ribosomal complexes. When cytoplasmic 20S pre-rRNA processing is impaired, as in Rio1p- or Nob1p-depleted cells, a large part of pre-SSUs is associated with translating ribosomes complexes. Loading of pre-40S particles onto mRNAs presumably uses the canonical pathway as translation-initiation factors interact with 20S pre-rRNA. However, translation initiation is not required for 40S ribosomal subunit maturation. We also provide evidence suggesting that cytoplasmic 20S pre-rRNAs that associate with translating complexes are turned over by the no go decay (NGD) pathway, a process known to degrade mRNAs on which ribosomes are stalled. We propose that the cytoplasmic fate of 20S pre-rRNA is determined by the balance between pre-SSU processing kinetics and sensing of ribosome-like particles loaded onto mRNAs by the NGD machinery, which acts as an ultimate ribosome quality check point. The EMBO Journal (2010) 29, 80-92. doi: 10.1038/emboj.2009.307; Published online 5 November 2009
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