Journal
EMBO JOURNAL
Volume 28, Issue 10, Pages 1418-1428Publisher
WILEY
DOI: 10.1038/emboj.2009.88
Keywords
ChIP-Seq; estrogen receptor alpha; RNA polymerase II; SERMs
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Funding
- Radboud University Nijmegen Medical Centre
- Radboud University Science Faculty
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We used ChIP-Seq to map ER alpha-binding sites and to profile changes in RNA polymerase II (RNAPII) occupancy in MCF-7 cells in response to estradiol (E2), tamoxifen or fulvestrant. We identify 10 205 high confidence ER alpha-binding sites in response to E2 of which 68% contain an estrogen response element (ERE) and only 7% contain a FOXA1 motif. Remarkably, 596 genes change significantly in RNAPII occupancy (59% up and 41% down) already after 1 h of E2 exposure. Although promoter proximal enrichment of RNAPII (PPEP) occurs frequently in MCF-7 cells (17%), it is only observed on a minority of E2-regulated genes (4%). Tamoxifen and fulvestrant partially reduce ER alpha DNA binding and prevent RNAPII loading on the promoter and coding body on E2-upregulated genes. Both ligands act differently on E2-downregulated genes: tamoxifen acts as an agonist thus downregulating these genes, whereas fulvestrant antagonizes E2-induced repression and often increases RNAPII occupancy. Furthermore, our data identify genes preferentially regulated by tamoxifen but not by E2 or fulvestrant. Thus (partial) antagonist loaded ER alpha acts mechanistically different on E2-activated and E2-repressed genes. The EMBO Journal (2009) 28, 1418-1428. doi:10.1038/emboj.2009.88; Published online 4 April 2009
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