Journal
EMBO JOURNAL
Volume 27, Issue 9, Pages 1309-1320Publisher
WILEY
DOI: 10.1038/emboj.2008.72
Keywords
hair follicle stem cells; immortal strand hypothesis; label retaining cells; proliferation history
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Funding
- NIAMS NIH HHS [AR053201, R01 AR053201, R56 AR053201] Funding Source: Medline
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Regulation of stem cell (SC) proliferation is central to tissue homoeostasis, injury repair, and cancer development. Accumulation of replication errors in SCs is limited by either infrequent division and/or by chromosome sorting to retain preferentially the oldest 'immortal' DNA strand. The frequency of SC divisions and the chromosome-sorting phenomenon are difficult to examine accurately with existing methods. To address this question, we developed a strategy to count divisions of hair follicle (HF) SCs over time, and provide the first quantitative proliferation history of a tissue SC during its normal homoeostasis. We uncovered an unexpectedly high cellular turnover in the SC compartment in one round of activation. Our study provides quantitative data in support of the long-standing infrequent SC division model, and shows that HF SCs do not retain the older DNA strands or sort their chromosome. This new ability to count divisions in vivo has relevance for obtaining basic knowledge of tissue kinetics.
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