Journal
EMBO JOURNAL
Volume 27, Issue 21, Pages 2896-2906Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/emboj.2008.205
Keywords
chromosomal fragility; chromosomal rearrangements; DNA secondary structure; mismatch repair; trinucleotide repeats
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Funding
- NIGMS/NIH [R01GM0825950, R01GM52319, R01GM60987]
- FARA
- MDA foundations
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Expansion of triplex-forming GAA/TTC repeats in the first intron of FXN gene results in Friedreich's ataxia. Besides FXN, there are a number of other polymorphic GAA/TTC loci in the human genome where the size variations thus far have been considered to be a neutral event. Using yeast as a model system, we demonstrate that expanded GAA/TTC repeats represent a threat to eukaryotic genome integrity by triggering double-strand breaks and gross chromosomal rearrangements. The fragility potential strongly depends on the length of the tracts and orientation of the repeats relative to the replication origin, which correlates with their propensity to adopt triplex structure and to block replication progression. We show that fragility is mediated by mismatch repair machinery and requires the MutS beta and endonuclease activity of MutL alpha. We suggest that the mechanism of GAA/TTC-induced chromosomal aberrations defined in yeast can also operate in human carriers with expanded tracts.
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