Journal
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
Volume 197, Issue -, Pages 38-49Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.aanat.2014.10.006
Keywords
PRP; Rat model; Osteoarthritis; Synovium; PDGF; VEGF
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Funding
- Taibah University, Almadinah Almonawarah, Kingdom of Saudi Arabia [929/1433]
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This study was carried out on a rat model of surgically-induced osteoarthritis (OA) to assess the histological and immunohistochemical changes in the synovial membrane and to evaluate the effects of intra-articular injection of platelet rich plasma (PRP) in such cases. Forty five male albino rats were divided into 3 equal groups; control, surgically-induced OA and surgically-induced OA followed by intra-articular injection of PRP. Knee joints were processed for histological and immunohistochemical staining with anti-platelet derived growth factor (PDGF-A) and anti-vascular endothelial growth factor (VEGF) and the area percentages of immunostaining were measured by digital image analysis. Serum levels of PDGF-A and VEGF were analyzed by ELISA. The osteoarthritis research society international (OARSI) score was significantly higher in OA (2433.8 +/- 254) than in control (230.4 +/- 37.8; p < 0.001) and in PRP-treated tissues (759.7 +/- 45.8; p < 0.001). The immunostained area percentages for PDGF-A was significantly higher in PRP-treated tissues (20.6 +/- 2.4) than in OA (11.06 +/- 1.3; p = 0.007) and in control tissues (4.1 +/- 0.78; p < 0.001). Likewise, the immunostained area percentage for VEGF was significantly higher in PRP-treated tissues (22.5 +/- 1.6) than in OA (14.9 +/- 1; p = 0.002) and in control tissues (6.5 +/- 0.7; p < 0.001). ELISA analysis revealed a significant increase in serum levels of the PDGF-A and VEGF after intraarticular PRP injection when compared to the other groups (p < 0.000). The present study concluded that intra-articular injection of PRP could produce optimizing effects in surgically induced OA in the form of; decreasing the OARSI score, improving the inflammatory events in synovium and modulating the PDGF-A and VEGF serum levels and synovial tissue immunoexpression. These effects could be reflected positively on the associated chondral defect. (C) 2014 Elsevier GmbH. All rights reserved.
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