Journal
ELECTROPHORESIS
Volume 35, Issue 16, Pages 2325-2332Publisher
WILEY
DOI: 10.1002/elps.201300511
Keywords
Amperometry; CE; CO2-laser; Engraving; Microchips
Funding
- University of Texas at San Antonio
- National Institutes of Health through the National Institute of General Medical Sciences [2SC3GM081085]
- Research Centers at Minority Institutions [G12MD007591]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [246903/2012-0]
- Instituto Nacional de Ciencia e Tecnologia de Bioanalitica (Science without Borders Program)
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This paper describes the effects of different modes and engraving parameters on the dimensions of microfluidic structures produced in PMMA using laser engraving. The engraving modes included raster and vector, while the explored engraving parameters included power, speed, frequency, resolution, line-width, and number of passes. Under the optimum conditions, the technique was applied to produce channels suitable for CE separations. Taking advantage of the possibility to cut-through the substrates, the laser was also used to define solution reservoirs (buffer, sample, and waste) and a PDMS-based decoupler. The final device was used to perform the analysis of a model mixture of phenolic compounds within 200 s with baseline resolution.
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