Journal
ELECTROPHORESIS
Volume 35, Issue 11, Pages 1728-1734Publisher
WILEY
DOI: 10.1002/elps.201300559
Keywords
Food adulteration; Microchip capillary electrophoresis; Soybean protein
Funding
- Hong Kong Research Grants Council of the Hong Kong Special Administrative Region, China [HKU7023/07P]
- Hong Kong University Research and Conference Grants Committee
- Singapore Institute of Manufacturing Technology, under the Agency for Science, Technology and Research (AstarSTAR, Singapore)
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To determine the adulteration of soybean proteins in dairy product, a microchip-CE device was developed to isolate selected fraction of soybean and milk proteins in pI range from 5.5 approximate to 7.0 by 1D IEF, followed by ITP/CZE in the embedded capillary for preconcentration, separation and UV detection at 280 nm. Compared to IEF-CZE without ITP preconcentration, the enhancement factor (EF) in detection of soybean proteins was 20times. Adulteration of 0.1% soybean protein in total dairy proteins can be detected in less than 10 min.
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