Journal
ELECTROPHORESIS
Volume 35, Issue 7, Pages 967-971Publisher
WILEY-BLACKWELL
DOI: 10.1002/elps.201300450
Keywords
Capillary electrophoresis; Erythrocytes; Glutathione
Funding
- Louisiana State University Health Sciences Center Department of Pediatrics
- Research Institute for Children at Children's Hospital of New Orleans
Ask authors/readers for more resources
This report describes modifications to a CZE method developed by Serru et al. (Clinical Chemistry 2001, 47, 1321-1324) for the simultaneous analysis of reduced glutathione (GSH) and glutathione disulfide (GSSG). Lowering the pH of the run buffer (75 mmol/L boric acid, 25 mmol/L bis-Tris) from pH 8.4 to 7.8 markedly improved GSH peak area reproducibility and allowed multiple samples to be analyzed without changing run buffers due to ion depletion. Sample preparation using red blood cells (RBC) instead of whole blood, combined with glutathione extraction at a lower concentration of metaphosphoric acid (5%), increased assay sensitivity and decreased interference. CZE assay results for clinical samples containing 1000 to 3200 mol GSH/L RBC and 100 to 400 mol GSSG/L RBC were highly correlated (r(2) 0.95) with results obtained using a commercial dithionitrobenze-based glutathione assay. The modified CZE assay has proven useful for the analysis of glutathione in both mouse and human RBC.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available